Bicinchoninic Acid Protein Assay:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
Quantitative RT-PCR:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
Crocin Bleaching Assay:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
Enzyme-linked Immunosorbent Assay:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
Sequencing:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
Software:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++. .. Boston BioProducts Cat# IBB-300X EDTA Thermo Fisher Scientific Cat# AM9260G BD GolgiPlug BD Biosciences Cat# 555029 BD GolgiStop BD Biosciences Cat# 554724 Zombie Violet Fixable Viability Kit Biolegend Cat# 423113 Penicillin-Streptomycin Gibco Thermo Fisher Scientific Cat# 15140122 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Quick-RNA Viral 96 Kit Zymo Research Cat# R1040 LUNA Universal Probe One-Step RT-qPCR Kit New England BioLabs Cat# E3005S BD Human Inflammatory Cytokine Bead Array (CBA) BD Biosciences Cat# 551811 C-Reactive Protein ELISA (CRP ELISA) ALPCO Diagnostics Cat# 30-9710S Monkey anti-nuclear IgM kit Alpha Diagnostic International Cat# 670-115-ANM Monkey anti-nuclear IgG kit Alpha Diagnostic International Cat# 670-110-ANM Experimental models: Cell lines MDCK-SIAT1-PB1 NIH Vaccine Research Center 59 N/A KHYG-1 MmCD16 Evans laboratory 64 N/A THP-1 ATCC (via Cell Engineering Shared Resource Lab, WFUSM) Cat# TIB-202 Experimental models: Organisms/strains Six-week-old female BALB/c Charles River Laboratories Cat# 028 African Green Monkeys Vervet Research Colony – Wake Forest University School of Medicine N/A Oligonucleotides InfA For1 5 ′ -CAAGACCAATCYTGTCACCTCTGAC-3 ′ CDC designed Integrated DNA Technologies InfA For2 5 ′ -CAAGACCAATYCTGTCACCTYTGAC-3 ′ CDC designed Integrated DNA Technologies InfA Rev1 5 ′ -GCATTYTGGACAAAVCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Rev2 5-′ GCATTTTGGATAAAGCGTCTACG-3 ′ CDC designed Integrated DNA Technologies InfA Probe 5 ′ -/5FAM/TGCAGTCCT/ ZEN/CGCTCACTGGGCACG/3IABkFQ/-3 ′ CDC designed Integrated DNA Technologies Synthetic DNA target sequence: cgactaatacgactcac tatagggagaCAAGACCAATCTTGTCACCTCTGACTA AGGGAATTTTAGGATTTGTGTTCACGCTCACCGTG CCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTA TCCAAAATGCaacatttacagcTCCTCAACTCACTCTTC GAGCGTCTCAATGAAGGACATTCAAAGCCAATTCG AGCAGCTGAAACTGCGGTGGGAGTCTTATCCCAA TTTGGTCAAGAGCACCGCttctatagtgtcacctaaatggatct Integrated DNA Technologies GenBank Accession # MN976418.1, A/Hawaii/66/2019[H1N1], segment 7, matrix gene Software and algorithms ZetaSizer Software Malvern Panalytical USB0048 DigitalMicrograph Software Gatan https://www.gatan.com/ installation-instructions GraphPad Prism Software GraphPad https://www.graphpad.com (Continued on next page) Cell Reports Medicine 7, 102746, May 19, 2026 e2 OPEN ACCESS .. Six-week-old female BALB/c (strain code 028) mice were obtained from Charles River Laboratories.
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